By Ulrich Müller Charles E. Mortimer. Show description. Read or Download Chemie PDF. Similar chemistry books. Get Advances in Free Radical Chemistry, . 1. Mai Standardwerk für Erstsemester: Das Basiswissen der Chemie. Von Charles E. Mortimer. Thieme Verlag, Stuttgart , Seiten, brosch., GENERAL CHEMISTRY CHARLES MORTIMER 6TH EDITION PDF - site S3 download Chemie: Das Basiswissen der Chemie by Ulrich Müller Charles E.
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For each method, different concentrations of the extracts were tested. The concentrations capable of providing a reasonable kinetic curve were employed for determining the antioxidant capacity of the samples. Next, the volume of 1.
The readings were performed at different time intervals 0, 15 and at every 30 sec during a period of 6 min. The absorbance readings were corrected by the control with no antioxidants addition. The change in the optical density reading of the samples was correlated to the standard and the FRAP values mmol of the samples were established as shown below.
All the analysis findings were compared with the control no antioxidant addition. The pH of the buffer made in this manner should be around 7.
However, the pH should be checked using a pH meter. Then 1. The volume of ml Trolox, in a concentration range from 6 mmol to 20 mmol, was used for calibration.
The last reading was correlated to the first, and the area under the curve AUC was established for the samples and for the standard. The results obtained by the three methods are presented in Table I.
Antioxidant capacity of the sunflower seed extracts 5 Table I. Antioxidant capacity of the ethanolic and aqueous extracts of the striped sunflower seed cotyledon and of BHT. The results obtained by the FRAP assay showed that the sequential extraction with different polarities of solvents provided compounds of different antioxidant capacities for the studied extracts.
In our other previously mentioned study Giada , we found that this extract has a high content of phenolic compounds, especially chlorogenic acid However, caffeic acid 1. The p-hydro- xybenzoic acid 0. This way, the electron-donating antioxidants of the aqueous extract from the striped sunflower seed are probably these phenolic substances.
By applying the FRAP assay for determining the sample antioxidant total values, these authors also found a high content of antioxidant compounds in the sunflower seeds 5. Additionally, during the FRAP assay we verified different absorbance readings among the samples at the zero time of reaction. However, the results of the FRAP assay showed that the antioxidant capacity of the striped sunflower seed aqueous extract was higher than the ethanolic extract in all tested concentrations.
Nevertheless, between the extracts, it was the aqueous extract that showed the highest 6 M.
Our results for the DPPH assay are somewhat similar to the findings of such studies, and show that this method is simple and effective for antioxidant capacity evaluation, as found by other existing assays carried out for the same purpose. Table I also shows that the results of the ORAC assay are in conformity with those found by applying the other two methods. Therefore, between the extracts, it was the aqueous extract that also had the highest antioxidant capacity in the ORAC assay.
There are few studies applying the ORAC assay to evaluate the antioxidant capacity of oilseeds Aldini et al. However, our findings using the ORAC method were similar to these studies and showed that this assay is also effective for antioxidant capacity evaluation. As mentioned before, it is necessary to apply different methods to evaluate the antioxidant capacity.
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Back to overview. Loading sales information. Academic Editor: James Krzanowski. Received: Mortimer, C. Mortimer, Charles E. Release of aluminium and thallium ions from uncoated food Mortimer UM.
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